BMY3102 MICROBIOLOGY II
WHAT HAVE I LEARN?
We continue on the journal about the how to culture a fungi. Dr go through all our flow chart group by group. Dr give comment on all the flow chart and correct some mistakes which frequently done by all of us. The flow chart must include title and also the source of references. Many of us didn't write the title of flow chart. Our group get praise from Dr as we done the flow chart by referring on a journal.Next, we are given time to comment on other's group flow chart. We can ask anything that we don't know and what we don't understand. Dr then go through the comment and give more explanation and answer. After that, Dr go through about fungi and algae on the notes. Before end of the class, we are advised to do revision on the topic of fungi and algae for the quiz on friday. We have to find the journal article about how to isolate virus.
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-8-1927#tab2
This is the link of journal article that I found. It is about isolation of Hendra virus from pteropid bats: a natural reservoir of Hendra virus. Some of our classmate didn't upload the journal article on padlet. We are given time to upload the journal at least one of each person and choose the best article to convert into flow chart. In the end, my groupmate choose the article that i found. We read the article together and work into the flow chart. Along the isolation, we have to do the RNA isolation, PCR, nucleotide sequencing, IFA staining and others important steps. At the last 30 mins, Dr talk about some notes for next week topic.
Flow chart about how to isolate virus:
https://padlet-uploads.storage.googleapis.com/190839281/7735e2fb1f3a697044a76fe6942068f2/ISOLATION_OF_HENDRA_VIRUS_FROM_PTEROPID_BATS.pdf
Sunday, 4 March 2018
Sunday, 25 February 2018
Week 2
BMY3102 MICROBIOLOGY II
WHAT HAVE I LEARN?
This week we talk about the topic of fungi. We sit in groups and are given time to draw a mind map based on our knowledge without referring any lecture notes or our own mind map prepared before class. Within 10 minutes, we have to come out with a mind map. With the help of all the group members, we write in whatever notes that we remembered into a blank paper. However, the mind map is not said to be very complete as we forget some words and term. After 10 minutes, we swap our mind map with other groups. At this time, we will add on some notes that are haven't written in other group's mind map. After that, we correct some wrongly spelled term and improve the mind map. We swap again for second time and write on additional notes on other group's mind map. In the end, we get back our own mind map with full of information and notes. The mind map becoming more complete and can be use as reference when doing revision. From this activity, I know that preparing for class before attending the class is very important, and also able to contribute to the group. At the same time, we also know that team work is needed when come to study. We can learn from each other and share our knowledge together, and also improve together.
After that, we discuss about how to transfer a thermophilic fungi to culture in the lab. We can collect the fungi into a thermoflask and bring back to the lab. We are asked about the step on culturing the fungi in the lab. We cannot do streaking on the agar plate like the bacteria we culture before. We given time to write the steps of culturing a fungi in the lab. We work ourselves in group to find some journal article from google.
Journal article: http://www.mdpi.com/2309-608X/3/3/36/pdf
WHAT HAVE I LEARN?
This week we talk about the topic of fungi. We sit in groups and are given time to draw a mind map based on our knowledge without referring any lecture notes or our own mind map prepared before class. Within 10 minutes, we have to come out with a mind map. With the help of all the group members, we write in whatever notes that we remembered into a blank paper. However, the mind map is not said to be very complete as we forget some words and term. After 10 minutes, we swap our mind map with other groups. At this time, we will add on some notes that are haven't written in other group's mind map. After that, we correct some wrongly spelled term and improve the mind map. We swap again for second time and write on additional notes on other group's mind map. In the end, we get back our own mind map with full of information and notes. The mind map becoming more complete and can be use as reference when doing revision. From this activity, I know that preparing for class before attending the class is very important, and also able to contribute to the group. At the same time, we also know that team work is needed when come to study. We can learn from each other and share our knowledge together, and also improve together.
After that, we discuss about how to transfer a thermophilic fungi to culture in the lab. We can collect the fungi into a thermoflask and bring back to the lab. We are asked about the step on culturing the fungi in the lab. We cannot do streaking on the agar plate like the bacteria we culture before. We given time to write the steps of culturing a fungi in the lab. We work ourselves in group to find some journal article from google.
Journal article: http://www.mdpi.com/2309-608X/3/3/36/pdf
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| Our mind map after improvement 😃 |
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| New sem new groupmate 🙂 |
Friday, 22 December 2017
WEEK 14
BMY3101-1 MICROBIOLOGY I
WHAT HAVE I LEARN?
This week is the last week of the sem 1 and also the week of "3MT". 3MT is a 3 minute talks which everyone of us have to present in front of our friend to tell them about our adopted microbes. I choose the microbes which are contributed in the biotechnology, which is Clostridium acetobutylicum. At the same time, we have to evaluate our classmate according to their performance. It was really nervous for me to talk in front and I have to stand on the stage somemore. But it was really a great experience to share my adopted microbes and my finding about this microbes to everyone. Below are my one slide for my presentation. On the next day which is the last class for microbiology I, we have small party. Classmate bring some snacks and also cakes from Dr. THANK YOU DR 💕 ! And we had our group photos! See you guys next sem !!!
Monday, 18 December 2017
WEEK 13
BMY3101-1 MICROBIOLOGY I
WHAT HAVE I LEARN?
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| Thanks Lokman for this ugly picture HAHAHA |
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| Poster from GROUP 14 |
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| Group photo 😏 |
Sunday, 10 December 2017
WEEK 12
BMY3201-1 BASIC MICROBIOLOGY TECHNIQUES
WHAT HAVE I LEARN?
This week is the last experiment conducting for the sem 1. We doing on the Experiment 18, Serial Dilution- Agar Plate Procedure to Quantitate Viable Cells. There are many methods in taking measurement of quantity of microbes in particular medium, including direct microscopic counts, electronic cells counter, chemical method, spectrophotometric analysis and also the Agar Plate Analysis. However the best way to gets the number of colonies for viables cells is through the Agar Plate Analysis.
Greater manipulation are needed for doing this experiment, we must be always clear on the dilution of the E. coli culture. This method involve serial dilution which the bacterial suspension in sterile water blanks. After dilution have done, the culture will be transfer to Petri dish by using pour plate technique or spread plate technique. For pour plate technique, the cooled Molten agar of 45°C is added to the Petri dish containing 1.0ml of bacterial suspension. The Petri dish is then gently rotated in a circular motion to make sure that all the bacterial cells are evenly distributed. For spread plate technique, the 0.1ml of bacterial suspension is carefully added into the Petri dish containing pre-prepared agar nutrient and is rotated using L-shaped glass rod in a circular motion. The average count per ml of sample is taken for both spread plate and pour plate. The number of organisms per ml of original culture is calculated by multiplying the number of colonies counted by the dilution factor:
WHAT HAVE I LEARN?
This week is the last experiment conducting for the sem 1. We doing on the Experiment 18, Serial Dilution- Agar Plate Procedure to Quantitate Viable Cells. There are many methods in taking measurement of quantity of microbes in particular medium, including direct microscopic counts, electronic cells counter, chemical method, spectrophotometric analysis and also the Agar Plate Analysis. However the best way to gets the number of colonies for viables cells is through the Agar Plate Analysis.
Greater manipulation are needed for doing this experiment, we must be always clear on the dilution of the E. coli culture. This method involve serial dilution which the bacterial suspension in sterile water blanks. After dilution have done, the culture will be transfer to Petri dish by using pour plate technique or spread plate technique. For pour plate technique, the cooled Molten agar of 45°C is added to the Petri dish containing 1.0ml of bacterial suspension. The Petri dish is then gently rotated in a circular motion to make sure that all the bacterial cells are evenly distributed. For spread plate technique, the 0.1ml of bacterial suspension is carefully added into the Petri dish containing pre-prepared agar nutrient and is rotated using L-shaped glass rod in a circular motion. The average count per ml of sample is taken for both spread plate and pour plate. The number of organisms per ml of original culture is calculated by multiplying the number of colonies counted by the dilution factor:
Number of cells per ml= number of colonies x dilution factor
The Quebec colony counter and machanical hand counter is used in obtaining the number of colonies appear on the Petri dish. We can see that the bacterial colonies will grow on the surface and also suspended inside the nutrient medium for the pour plate technique. The Petri dish using spread plate technique only shows bacterial grow on the surface of agar.
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| This is Quebec colony counter. |
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| Final dilution of 10-7 |
WEEK 12
BMY3101-1 MICROBIOLOGY I
WHAT HAVE I LEARN?
This week we learn about the Acellular microorganisms, which is the viruses, viroid and prions. Viruses are more smaller than the bacteria and they can only view under electron microscope.
Virus Family can be categorized into DNA viruses and also RNA viruses.
For DNA viruses, DNA is released into the nucleus of the host cell. Transcription and translation produce viral DNA and later capsid protein which is synthesized in the cytoplasm.
For RNA viruses, RNA- dependent RNA polymerases synthesized the ds RNA. After maturation, viruses are released by budding or through ruptures in the host cell membrane.
Viruslike agents: viroids and prions
Viroids: infectious RNA particles smaller than a virus.
- consists of a single circular RNA molecule of low molecular weight
- exist inside cells as particles of RNA without capsids or envelope
- do not require a helper virus
- does not produce protein
- always copied in the host cell nucleus
- not apparent in infected tissues without the use of special techniques to identify nucleotide sequences in the RNA
Prions: with no nucleic acid component.
- heating to 90°C which inactivate virus
- not sensitive to radiation
- sensitive to protein denaturing agents such as phenol and urea
In class, we are given task to find a virus and look for it's characteristic, know more about that particular virus. For our group, we have chosen the HIV. The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome (AIDS). Without being on medications to stop HIV's replication, this process can take up to 10 years on average. To read more about HIV, go to https://padlet.com/myaaamin98/9ihbdgpduxzj 😏
WEEK 11
BMY3201-1 BASIC MICROBIOLOGY TECHNIQUES
WHAT HAVE I LEARN?
We are doing on the Experiment 16 and Experiment 17 this week, which are the physical factors: Atmospheric Oxygen Requirements and Technique for the Cultivation of Anaerobic Microorganisms.
Microorganisms can be classified into 5 major groups according to their needs for oxygen: Aerobes, microaerophiles, obligate anaerobes, aerotolerant anaerobes and facultative anaerobes. Aerobes microorganisms require oxygen for growth, the microaerophiles only require limited amount of oxygen where excess oxygen may inhibit activities of oxidative enzyme. Obligate anaerobes require other molecules but not oxygen as their final electron acceptor. Aerotolerant anaerobes are fermentative organisms which do not use any oxygen. Facultative anaerobes can grow in either present or absence of oxygen, but they prefer growing in the presence of oxygen. By comparing the distribution of growth for each microbes that are transferred into a melted agar deep tube after incubation, we can classify the microbes into different groups. In oxygen requirement classification, Micrococcus luteus is categorized under aerobic. Bacillus cereus and Escherichia coli are group members of facultative anaerobic. For Clostridium sporogenes, they group under obligate anaerobic.
For evacuation and replacement of oxygen atmosphere in sealed jar, we used GasPak system for Experiment 17. The evolved hydrogen gas is reacted with oxygen to yield water molecules. However, we cannot get the result for this part of experiment. This is because there are oxygen that entered the system. This may be cause by too much of Petri dish is put together at the same time in the system. Clostridium sporogenes should shows growth under anaerobic condition. We also used fluid thioglycollate medium to classify the microbes. If pink colour is present, which means that there is oxydized part. All the bacterial species that is Bacillus cereus, Escherichia coli, Micrococcus luteus and Clostridium sporogenes show growth in the fluid thioglycollate.
WHAT HAVE I LEARN?
We are doing on the Experiment 16 and Experiment 17 this week, which are the physical factors: Atmospheric Oxygen Requirements and Technique for the Cultivation of Anaerobic Microorganisms.
Microorganisms can be classified into 5 major groups according to their needs for oxygen: Aerobes, microaerophiles, obligate anaerobes, aerotolerant anaerobes and facultative anaerobes. Aerobes microorganisms require oxygen for growth, the microaerophiles only require limited amount of oxygen where excess oxygen may inhibit activities of oxidative enzyme. Obligate anaerobes require other molecules but not oxygen as their final electron acceptor. Aerotolerant anaerobes are fermentative organisms which do not use any oxygen. Facultative anaerobes can grow in either present or absence of oxygen, but they prefer growing in the presence of oxygen. By comparing the distribution of growth for each microbes that are transferred into a melted agar deep tube after incubation, we can classify the microbes into different groups. In oxygen requirement classification, Micrococcus luteus is categorized under aerobic. Bacillus cereus and Escherichia coli are group members of facultative anaerobic. For Clostridium sporogenes, they group under obligate anaerobic.
For evacuation and replacement of oxygen atmosphere in sealed jar, we used GasPak system for Experiment 17. The evolved hydrogen gas is reacted with oxygen to yield water molecules. However, we cannot get the result for this part of experiment. This is because there are oxygen that entered the system. This may be cause by too much of Petri dish is put together at the same time in the system. Clostridium sporogenes should shows growth under anaerobic condition. We also used fluid thioglycollate medium to classify the microbes. If pink colour is present, which means that there is oxydized part. All the bacterial species that is Bacillus cereus, Escherichia coli, Micrococcus luteus and Clostridium sporogenes show growth in the fluid thioglycollate.
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| Sterile brain heart infusion of C. sporogenes which shows evenly distrubution of growth through the gas producing |
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| M. luteus which produce pigmentation shows positive growth in aerobic condition |
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BMY3101-1 MICROBIOLOGY I WHAT HAVE I LEARN? This week is the last week of the sem 1 and also the week of "3MT". 3MT is a 3 ... -
BMY3101-1 MICROBIOLOGY I WHAT HAVE I LEARN? This week we move on to next topic that is the Introduction to Prokaryotes. We have cov... -
BMY3201-1 BASIC MICROBIOLOGY TECHNIQUES WHAT HAVE I LEARN? We are doing on the Experiment 16 and Experiment 17 this week, which are the p...